Elisa aims and method need to be paraphrase Essay

Elisa aims and method need to be paraphrase - Essay ExampleThis difference is made clear through the drawing of a standard curve, by making use of concentrations that are known belonging to antibodies of Rabbit immunoglobulin G.e. Incubation was done on different amounts of rabbit IgG. Micotiter plates were then cleaned by use of washing buffer, three times and washed again after blocking them with blocking buffer. They then were refrigerated, ready for use in the practical.1- For the monoclonal antibodies, the left field half of the plate got used.As in the illustration below in a picture with blue colour in the first column, 200ul of monoclonal purloin anti-rabbit IgG was added. 100ul of diluents buffer PBS was then filled to columns 2 and 6, for the monoclonal antibodies as in the below illustration.2- Then, 100ul of mouse anti-rabbit got transferred from A1 to A6 inorder to have the sample diluted and from well A6 100ul of mouse anti rabbit IgG antibody thrown away. A repeat of the process was done on the rest of the